At $7 million the item is the fourth-most expensive game-worn sports jersey behind Babe Ruth's "called shot" jersey ($24 million), Michael Jordan's 1998 NBA Finals jersey ($10.1 million) and Diego Maradona's Argentina shirt from the 1986 World Cup ($9.3 million).
"Debut games are truly one-of-a-kind moments in an athlete's career," Sotheby's head of modern collectibles Brahm Wachter said in a statement to ESPN.
The sale was a record for a Bryant collectible, surpassing the $5.85 million paid for his signed, game-worn 2007-08 MVP season jersey.
Five-time NBA champion Bryant died aged 41 in a January 2020 helicopter crash that also killed his daughter Gianna and seven others.